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Method of Determining Nanoparticie Core Weight.

Authors :
Reynolds, Fred
O'Loughlin, Terry
Weissleder, Ralph
Josephson, Lee
Source :
Analytical Chemistry. 2/1/2005, Vol. 77 Issue 3, p814-817. 4p.
Publication Year :
2005

Abstract

Polymer-coated metal or metal oxide nanoparticles have a variety of uses in industry, biological research, and medicine. Characterization of nanoparticles often includes determination of the dimensions of the electron-dense core by transmission electron microscopy (TEM), with the weight of the core determined from core volume and core density. However, TEM is labor intensive, has a long turnaround time, and uses equipment that is sometimes not readily available. Here we present an alternative method for determining the weight of nanoparticle cores termed the viscosity/light scattering method, which uses (i) measurements of viscosity over a wide concentration range to obtain the partial specific volume, (ii) measurements of particle diameter by light scattering, to obtain the volume of an individual particle, and (iii) the concentration of nanoparticles (w/v). We have applied this method to determine the weights of nanoparticle cores (iron of amino-CUO and ferritin), the weights of globular proteins (molecular weight of IgG and albumin), and the weight of polystyrene microspheres. The viscosity/light scattering method is nondestructive of the sample and can be performed with a variety of materials on a route basis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032700
Volume :
77
Issue :
3
Database :
Academic Search Index
Journal :
Analytical Chemistry
Publication Type :
Academic Journal
Accession number :
16052203
Full Text :
https://doi.org/10.1021/ac049307x