N- 豆蔻酰化转移酶在乳腺癌组织中的表达及作用研究.

Objective: To study the expression of N-myristoyltransferase 1 (NMT1) and 2 (NMT2) in breast cancer tissues and their biological effects on breast cancer cells. Methods: The expression of NMT1 and NMT2 in 40 breast cancer tissues were detected by ELISA, and their expressions were confirmed by immunohistochemistry. Small RNA interference technology knocked down the expression levels of NMT1 and NMT2 in breast cancer cells MCF-7 and BT-474. CCK-8 and Transwell chamber transmembrane assays were used to detect the changes of cell proliferation and metastasis before and after NMT1 and NMT2 knockdown. Results: The expressions of NMT1 and NMT2 in breast cancer tissues of patients with lymph node metastasis and stage III/IV were significantly increased (P<0.01). Using CCK-8 detection, it was found that the proliferation activity of breast cancer cells BT-474 and MCF-7 after NMT1 or NMT2 knockdown for 48 h was significantly lower than that of control cells (P<0.0001). Transwell chamber penetration test showed that the number of transmembrane cells in the NMT1 or NMT2 knockdown group was significantly lower than that in the control group (P<0.01). Conclusion: NMT1 and NMT2 play a key role in the occurrence and development of breast cancer. Knockdown of NMT1 and NMT2 can weaken the proliferation and metastasis of Breast cancer cancer cells. Targeted inhibition of NMT1 and NMT2 is expected to become an important molecular target for intervention in breast cancer. [ABSTRACT FROM AUTHOR]