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Identification of novel totiviruses from the ascomycetous fungus Geotrichum candidum.

Identification of novel totiviruses from the ascomycetous fungus Geotrichum candidum.

Authors :
Khan, Haris Ahmed
Kondo, Hideki
Shahi, Sabitree
Bhatti, Muhammad Faraz
Suzuki, Nobuhiro
Source :
Archives of Virology. Dec2022, Vol. 167 Issue 12, p2833-2838. 6p.
Publication Year :
2022

Abstract

Mycoviruses are widely distributed across the kingdom Fungi, including ascomycetous yeast strains of the class Saccharomycetes. Geotrichum candidum is an important fungal pathogen belonging to Saccharomycetes and has a diverse host range. Here, we report the characterization of four new classical totiviruses from two distinct Geotrichum candidum strains from Pakistan. The four identified viruses were tentatively named "Geotrichum candidum totivirus 1, 2, 3a, and 3b" (GcTV1-3b). The complete dsRNA genomes of the identified totiviruses are 4621, 4592, 4576, and 4576 bp in length, respectively. All totivirus genomes have two open reading frames, encoding a capsid protein (CP) and an RNA-dependent RNA polymerase (RdRP), respectively. The downstream RdRP domain is assumed to be expressed as a CP-RdRP fusion product via -1 frameshifting mediated by a heptameric slippery site. Sequence comparisons and phylogenetic analysis showed that each of the discovered viruses belongs to a new species of the genus Totivirus in the family Totiviridae, with GcTV1 and GcTV3 (a and b strains) clustering in one subgroup and GcTV2 in another subgroup. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03048608
Volume :
167
Issue :
12
Database :
Academic Search Index
Journal :
Archives of Virology
Publication Type :
Academic Journal
Accession number :
160707135
Full Text :
https://doi.org/10.1007/s00705-022-05611-7