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Cloning, Isolation, and Properties of a New Recombinant Tannase from the Aspergillus niger Fungus.
- Source :
-
Applied Biochemistry & Microbiology . Dec2022, Vol. 58 Issue 9, p958-965. 8p. - Publication Year :
- 2022
-
Abstract
- A new recombinant Aspergillus niger tannase (tannin acyl hydrolase) produced by the Penicillium verruculosum fungus has been studied. A strain with a high level of extracellular tannase (TAN2) secretion (80% of the total extracellular protein) was obtained by cloning the tan2 gene (PDB Acc. no: MT828303) into the recipient strain. The tannase enzyme preparation degraded tannins in black tea extracts. TAN2 was isolated in homogeneous form using chromatographic methods; the enzyme had a high activity with respect to gallotannin (53 U/mg) and less activity against propyl gallate (4.7 U/mg). Homogeneous TAN2 showed temperature and pH optima of 45°C and 3.5, respectively. At a temperature of 50°C, TAN2 retained above 80% of its activity for 3 h; at 60°C about 75% of its activity for 90 min was preserved; at 70°C, the enzyme was completely inactivated within 10 min. Tannase was characterized by a high tolerance to NaCl, the activity with respect to gallotannin exceeded 50% of the initial value in solutions with a salt concentration of up to 5 M. The tannase activity was stimulated by Ca2+, Mg2+, Zn2+, Mn2+, Cu2+, Cd2+, and Pb2+ by 3–64%, and inhibited by 4‒65% in the presence of Co2+, Fe3+ and Fe2+ ions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ASPERGILLUS niger
*MOLECULAR cloning
*TANNINS
*TEA extracts
*PENICILLIUM
*FUNGI
Subjects
Details
- Language :
- English
- ISSN :
- 00036838
- Volume :
- 58
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Applied Biochemistry & Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 160839729
- Full Text :
- https://doi.org/10.1134/S000368382209006X