Multifaceted Bacillus spp. for the management of cotton bacterial blight caused by Xanthomonas citri pv. malvacearum.

• Endophytic Bacillus spp. reduced cotton bacterial blight disease. • Bacillus spp. inhibited pathogen in vitro and decreased disease incidence in vivo. • Bacillus spp. enhanced yields and growth of cotton plants. • B. amyloliquefaciens (ETL2) secreted number of antibacterial secondary metabolites. • Eight Anti-microbial peptide genes (AMPs) detected in B. amyloliquefaciens (ETL2) Bacterial antagonists against plant pathogens are widely exploited for the biological management of plant diseases. In the present study, antagonists were isolated from cotton plant associated bacteria such as endophytes (74 isolates), phylloplane (32 isolates) and cotton rhizosphere soils (48 isolates). Their efficacy against X. citri pv. malvacearum (Xcm) were evaluated in vitro by agar well diffusion technique. Among the isolates, the endophyte Bacillus amyloliquefaciens strain ETL2 was very effective in inhibiting the Xcm growth with an area of 983.76 mm2 followed by Bacillus velezensis strain ELS2 with an area of 944.41 mm2. PCR amplification of antimicrobial peptide (AMP) genes revealed that B. amyloliquefaciens strain ETL2 had maximum of 8 antibiotic biosynthesis genes namely, ItuC , bacA , bacD , bamC , fenCEA , sfrA , spaCS and mycC that produced antibiotics Iturin, bacilysin, bacillomycin, fengycin, surfactin, subtilin and mycosubtilin. Further, B. amyloliquefaciens strain ETL2 associated with Xcm inhibition was identified as aromatic hydrocarbons, phenols and fatty acids by Gas chromatography and mass-spectrometry (GC–MS) analysis of culture extracts. Delivery of B. amyloliquefaciens strain ETL2 and B. velezensis strain ELS2 as seed treatment and foliar spray was found promising for the management of bacterial blight of cotton in pot and field experiments. In pot and field experiments, the maximum plant growth promotion and seed cotton yield were observed in the plants treated with B. amyloliquefaciens strain ETL2 followed by B. velezensis strain ELS2 with better disease control efficiency compared to chemical control agents and chemical plant defense inducers. [ABSTRACT FROM AUTHOR]