Three-dimensional biomimetic reinforced chitosan/gelatin composite scaffolds containing PLA nano/microfibers for soft tissue engineering application.

In the current study, we successfully prepared chitosan/gelatin composite scaffolds reinforced by centrifugally spun polylactic acid (PLA) chopped nano/microfibers (PLA-CFs). Herein, different amounts of PLA-CFs (0 %, 1 %, 2 %, 3 %, and 4 % w / v) dispersed in chitosan/gelatin solution were used. Morphological characterization of prepared scaffolds revealed that at the initial stage of adding PLA-CFs, the chopped fibers were localized at the wall of the pores; however, as the fiber load increased, aggregations of chopped-fibers could be seen. Also, mechanical evaluation of scaffolds in terms of compression and tensile mode showed that samples reinforced with 2 % PLA-CFs had enhanced mechanical properties. Indeed, its tensile strength increased from 123.8 to 247.2 kPa for dry and 18.9 to 48.6 kPa for wet conditions. Furthermore, the tensile modulus associated with both conditions increased from 2.99 MPa and 44.5 kPa to 6.43 MPa and 158.4 kPa, respectively. The results of cell culture studies also confirmed that the prepared composite scaffold exhibited appropriate biocompatibility, cell proliferation and migration. The cell infiltration study of the samples revealed that scaffolds reinforced with 2 % PLA-CFs had significantly better cell penetration and distribution compared with the control ones on both days (7 and 14). [Display omitted] • Short PLA nano/micro fibers were obtained using centrifugal spinning/milling method. • Chopped PLA nano/micro fibers were dispersed inside the chitosan/Gelatin hydrogel. • Adding centrifugal-spun nano/micro fibers, enhanced mechanical properties of composite scaffolds specially at wet condition. • Prepared composite scaffolds revealed improved HDF-GFP+ cells bioactivity and infiltration. [ABSTRACT FROM AUTHOR]