Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats.

Sepsis, systemic inflammatory responses in western medicine stimulated by infections various sources and traditionally in Chinese medicine belong the poison heat syndrome. Vernodalin (VN) is a well-known cytotoxic sesquiterpene that has been renowned as anti-cancer, anti-inflammatory, and anti-bacterial properties. However, the molecular mechanism of VN on sepsis has not been studied. Therefore, we investigated the anti-inflammatory and antioxidant potential of VN against LPS-prompted sepsis in rats and also assess the impact of the NF-κBp65 signaling cascade. Rats were alienated into four groups: Group-I normal control, given 1 ml DMSO; while group-II rats were injected with LPS (100 μg/kg b.w., i.p.); Group-III rats were LPS (100 μg/kg b.w., i.p.) + VN (50 mg/kg b.w. in DMSO); Group- IV rats were given VN (50 mg/kg b.w. in DMSO) alone. We examined hepatic toxic markers, inflammatory cytokines, antioxidants, histopathology, and western blot analysis. Inflammatory markers, oxidative stress, and toxicity markers were significantly (p < 0.05) enhanced in the LPS-persuaded rats, whereas VN significantly (p < 0.05) prevented oxidative stress, lipid peroxides, toxic markers, pro-inflammatory cytokines, histological changes, and thereby multi-organ damage. VN significantly (p < 0.05) suppressed the NF-κβp65 signaling pathway and inhibited the Iκβα degradation. Thus, VN exerts a promising anti-septic natural agent for LPS-induced sepsis rats. [Display omitted] • Vernodalin (VN) is cytotoxic sesquiterpene possess numerous biological activities. • Anti-inflammatory potential of VN validated against LPS-prompted sepsis rats model. • VN prevented oxidative stress, toxic markers, pro-inflammatory cytokines. • VN suppressed the NF-κβp65 signalling pathway and prevented Iκβα degradation. • VN act as promising anti-septic natural agent for treating LPS-induced sepsis rats. [ABSTRACT FROM AUTHOR]