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Baculovirus LEF-11 interacts with BmIMPI to induce cell cycle arrest in the G2/M phase for viral replication.
- Source :
-
Pesticide Biochemistry & Physiology . Nov2022, Vol. 188, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- Viruses arrest the host cell cycle and using multiple functions of host cells is an important approach for their replication. Baculovirus arrests infected insect cells at both the late S and G2/M phase, but the strategy employed by baculovirus is not clearly understood. Our research suggests that the Bombyx mori nucleopolyhedrovirus (BmNPV) could arrest the cell cycle in the G2/M phase to promote virus replication, and also that the viral protein LEF-11 could inhibit host cell proliferation and arrest the cell cycle by inhibiting the cell cycle checkpoint proteins BmCyclinB and BmCDK1. Furthermore, we found that LEF-11 interacts with BmIMPI to regulate cell proliferation, but not by direct interaction with BmCyclinB or BmCDK1. In addition, our findings showed that BmIMPI was important and necessary for LEF-11 induced cell cycle arrest in the G2/M phase. Moreover, BmIMPI was found to interact with BmCyclinB and BmCDK1, and down-regulate the expression of BmCyclinB and BmCDK1 to compromise the cell cycle and cell proliferation. Taken together, the data presented demonstrated that baculovirus LEF-11 regulates BmIMPI to inhibit host cell proliferation and provide a new insight into the molecular mechanisms employed by viruses to induce cell cycle arrest. • Baculovirus LEF-11 hijacks BmIMPI and induces cell cycle arrest in the G2/M phase. • BmIMPI is a necessary host factor for LEF-11-induced cell cycle arrest. • Baculovirus LEF-11 depletes the BmCyclinB/CDK1 protein for viral replication. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00483575
- Volume :
- 188
- Database :
- Academic Search Index
- Journal :
- Pesticide Biochemistry & Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 161017776
- Full Text :
- https://doi.org/10.1016/j.pestbp.2022.105231