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Development and molecular characterization of doxorubicin-resistant canine mammary gland tumour cells.
- Source :
-
Journal of Applied Animal Research . Dec2022, Vol. 50 Issue 1, p125-145. 21p. - Publication Year :
- 2022
-
Abstract
- Canine mammary gland tumour (CMT) commonly affects the female dog. The objective of this study was to develop a doxorubicin-resistant CMT cell line and determine its in vitro and in vivo characteristics, including mRNA and microRNA (miRNA) expression profiles. Doxorubicin-resistant CMT-Star cells were developed from CMT-Stylo cells. The cells were characterized, including tumorigenicity in NOD/SCID mouse models. MiRNA and mRNA expression of the two cell lines were profiled and clustered. ATP binding cassette subfamily B member 1 (ABCB1) and subfamily G member 2 (ABCG2) expressions were significantly increased in the CMT-Star cell line. CMT-Star cells also had altered expression of 785 genes and 14 miRNAs. Downregulating plasminogen (PLG) and plasminogen activator urokinase (PLAU) while upregulating transforming growth factor beta receptor 3 (TGFBR3), epidermal growth factor receptor 1 (EGFR1) and ABCB1 rendered CMT-Star cells less proliferative, less invasive and more resistant to chemotherapeutic drugs. The upregulated miRNAs in CMT-Star cells include miRNA-191, -29a, -107, -99b, -874, -93 and -210, while the downregulated miRNAs include miRNAs-106a, -92a, -92b, -155 and -15b. TGFβR, EGF receptor 1 and Wnt signalling are enriched in doxorubicin-resistant CMT-Star cells and could be potential therapeutic targets in dogs with doxorubicin-resistant CMT. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09712119
- Volume :
- 50
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Applied Animal Research
- Publication Type :
- Academic Journal
- Accession number :
- 161031222
- Full Text :
- https://doi.org/10.1080/09712119.2022.2032719