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Comparative study on the extraction efficiency, characterization, and bioactivities of Bletilla striata polysaccharides using response surface methodology (RSM) and genetic algorithm-artificial neural network (GA-ANN).
- Source :
-
International Journal of Biological Macromolecules . Jan2023, Vol. 226, p982-995. 14p. - Publication Year :
- 2023
-
Abstract
- This research established the optimal conditions for alkali-assisted extraction (AAE) of bioactive polysaccharides from Bletilla striata integrated with response surface methodology (RSM) and the genetic algorithm-artificial neural networks (GA-ANN). In comparison with RSM, the ANN model showed a relatively higher determination coefficient in the global output values (RSM: ANN = 0.9270: 0.9742) performing more satisfactorily in the validation. Under the optimum conditions (52 °C; 167 min, and 0.01 mol/L NaOH), the extraction yields, IC 50 of ABTS, and FRAP value were 29.53 ± 0.97 %, 3.41 mg/mL, and 39.11 μmol Fe2+/g, respectively. The results indicated that BSPs-A was mainly composed of glucose and mannose with small amounts of arabinose, galactose, and galacturonic acid, while possessed a molecular weight of about 305.94 kDa (Mw). The structural characterization of BSPs-A was initially characterized by FT-IR, SEM, and Congo red tests, which indicated that BSPs-A possessed a triple helix conformation of typical Bletilla striata polysaccharides. In addition, BSPs-A exhibited excellent antioxidant activity, which was further confirmed by a series of in vitro antioxidant activity assays including DPPH, ABTS, FRAP, and ORAC. After incubation in the BSA-glucose system for 15 days, BSPs-A showed inhibition of the advanced glycation end products (AGEs) formation for the first time. [Display omitted] • The extraction of BSPs was optimized and predicted by RSM and GA-ANN models. • The extraction yield of BSPs-A was up to 29.53 % under the optimum conditions. • BSPs-A exhibited excellent antioxidant and anti-glycation activity. • ANN had a higher determination coefficient (0.9742) than RSM for global optimization. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 226
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 161058775
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2022.12.017