An enhanced chemiluminescence hybrids of luminol by sulfonated polyaniline decorated copper-based metal organic frame composite applicable to the measurement of hydrogen peroxide in a wide pH range.

Here, sulfonated polyaniline (SPAN) was decorated on the surface of copper-based metal organic frame (HKUST-1) and the composite was functionalized by luminol to construct a chemiluminescence (CL) hybrids (SPAN/HKUST-1@Luminol). The as-prepared SPAN/HKUST-1@Luminol demonstrated a great dispersion and stability performance in aqueous solution. Moreover, the resultant SPAN/HKUST-1@Luminol hybrids exhibited extremely strong CL properties, and the CL quantum yield was 136 times higher than that of luminol. In particular, it exhibited outstanding CL activity not only under alkaline conditions, but also under neutral conditions. The sensitive response of the hybrid to hydrogen peroxide was used to construct CL methods for the detection of hydrogen peroxide at a wide range of pH, with the detection limit of 60 nM at a neutral condition and 25 pM at alkaline condition. Due to strong and stable signal of the SPAN/HKUST-1@Luminol, the CL method provides a viable tool for determination of H 2 O 2 in biological systems and enabled the monitoring of stimulated production of H 2 O 2 released by living cells. SPAN/HKUST-1@Luminol CL hybrid exhibited extremely strong CL properties and the CL signal maintained over a wide range of pH ranges. The resultant CL composite was applied as the probe to directly detect the amount of hydrogen peroxide released after stimulation of living cells under physiological conditions. [Display omitted] • A novel CL hybrid was constructed with luminol attached to the composite of SPAN and HKUST-1. • The as-prepared SPAN/HKUST-1@Luminol demonstrated a great dispersion and stability performance in aqueous solution. • The SPAN/HKUST-1@Luminol hybrid exhibited great luminous efficiency under a wide range of pH conditions. • The CL probe enabled the monitoring of stimulated production of H2O2 released by living cells. [ABSTRACT FROM AUTHOR]