Effect of acute vs chronic stress on Polyhydroxybutyrate production by indigenous cyanobacterium.

Fossil-based plastic has become a global-threat due to its high stability and transformation into more lethal forms such as micro plastics with time. An alternative should be found to combat this global enemy. Polyhydroxybutyrate or PHB can be such an alternative to plastic. Present study explores the synthesis of PHB in Neowollea manoromense , using two different cultivation-approaches: acute and chronic stress. This study has used 6 carbon sources and 3 different level of phosphate to study the accumulation of PHB along with lipid, carbohydrate, and proteins. Highest PHB in chronic-stress was achieved under glucose supplementation without phosphate at 21st day (156.5 ± 22.5 μg/mg), whereas in acute-stress, it was achieved under acetate without phosphate (91.0 ± 2.7 μg/mg). Despite higher accumulation in chronic-stress, high PHB productivity was achieved in acute-stress. Principal Component Analysis suggests that all the variables were positively correlated with each other. Here we first report PHB accumulation in Neowollea manoromense. This study highlights that acute-stress can be a powerful tool in establishment of a sustainable cyanobacteria based bio refinery for PHB production. • First report for PHB accumulation in Neowollea manoromense • Acute vs chronic stress study • Effect of mixotrophic growth coupled with phosphate supplementation • 3.7-fold increase in productivity in acute stress • Sustainable approach for cyanobacterial biorefinery [ABSTRACT FROM AUTHOR]