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Improvement of Tol2 Transposon System by Modification of Tol2 Transposase.
- Source :
-
Biotechnology & Bioprocess Engineering . Dec2022, Vol. 27 Issue 6, p987-994. 8p. - Publication Year :
- 2022
-
Abstract
- Tol2 transposon-based vector platform has been developed as a useful strategy to improve protein yield. Tol2 transposase identifies inverted terminal repeats of transposons and enhances transgene integration efficiency in the genome. However, Tol2 transposon system does not reach a level of efficiency for use in commercial production, limiting its usefulness for mass production of recombinant proteins. Here, we proposed a novel strategy to improve the existing Tol2 transposase by adding a nuclear localization signal of histone H2B next to the Tol2 transposase (i.e., NLS-H2B-Tol2 transposase). Comparing the NLS-H2B-Tol2 transposase with the traditional Tol2 transposase, a significant increase in protein production was measured. Concurrently, a significant increase in nuclear transport of NLS-H2B-Tol2 transposase was observed, suggesting a causal relationship between increased nuclear transport and increased protein production. Furthermore, importin beta maximized NLS-H2B-Tol2-mediated protein productivity. Taken together, our findings offer useful ways for improving conventional Tol2 transposon-based vector platform. This new platform will be a breakthrough in the field of biopharmaceuticals to produce therapeutic proteins. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 12268372
- Volume :
- 27
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Biotechnology & Bioprocess Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 161192505
- Full Text :
- https://doi.org/10.1007/s12257-022-0175-9