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Blue honeysuckle rich in cyanidin-3-O-glucoside inhibited adipogenic differentiation by modulation of the adipogenesis pathway in 3T3-L1 adipocytes.
- Source :
-
Food & Nutrition Research . 2022, Vol. 66 Issue 2022, p1-11. 11p. - Publication Year :
- 2022
-
Abstract
- Background: Blue honeysuckle (BH; Lonicera caerulea L.), which is a traditional medicinal plant, is known to be a rich source of anthocyanins and phenolic acids due to its strong antioxidant and anti-inflammatory activities. Its anti-obesity effects, which are a result of attenuating abnormal lipid and glucose metabolisms, have also been reported. Aim: The purpose of this study is to investigate the effect of BH on genes and proteins that are involved in the adipocyte differentiation using 3T3-L1 cells. Methods: The effects of the water extracts of the BH were examined on adipogenesis and lipolysis using a biochemical and molecular analysis of the 3T3-L1 cells. Results: Cyanidin-3-O-glucoside (C3G) from the BH extract was determined in order to contain 1.67 mg/g by the high-performance liquid chromatography analysis. The lipid accumulation in the adipocytes was reduced, which ranged from 58 to 26% in the BH (500 and 1,000 µg/mL) compared to the control group. The lipolysis that was measured by the glycerol content was not affected by the BH at 1,000 μg/mL. The BH downregulated the expression of the main transcription factors of the adipogenesis pathway, such as peroxisome proliferator-activated receptor γ, 1, 2, adipose differentiation-related protein, CCAAT/enhancer binding protein α, and acetyl CoA carboxylase, while increasing the expression of the Adenosine monophosphate (AMP)-activated protein kinase α. Conclusion: These findings suggest that the BH is a good source of C3G, and it could be effective in regard to inhibiting the adipogenesis as opposed to the lipolysis, which indicates the potential for natural anti-obesity ingredients. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL differentiation
*PROTEIN kinases
*MEDICINAL plants
*CATTLE
*PHENOLS
*HIGH performance liquid chromatography
*ANIMAL experimentation
*INFLAMMATION
*GLYCOSIDES
*CELL physiology
*PEROXISOME proliferator-activated receptors
*CELLULAR signal transduction
*OXIDATIVE stress
*GENE expression
*FAT cells
*DESCRIPTIVE statistics
*CELL lines
*PLANT extracts
*TRANSCRIPTION factors
*BIOLOGICAL pigments
*LIPIDS
*CARRIER proteins
*ADENOSINE monophosphate
Subjects
Details
- Language :
- English
- ISSN :
- 16546628
- Volume :
- 66
- Issue :
- 2022
- Database :
- Academic Search Index
- Journal :
- Food & Nutrition Research
- Publication Type :
- Academic Journal
- Accession number :
- 161230328
- Full Text :
- https://doi.org/10.29219/fnr.v66.8501