Fbxo2CreERT2: A new model for targeting cells in the neonatal and mature inner ear.

• Fbxo2CreERT2 provides a new tool for manipulating gene expression the inner ear. • Recombination occurs throughout the cochlear duct in the neonatal and adult inner ear. • Hair cells in the neonatal vestibular end organs are targeted by Fbxo2CreERT2. • Fbxo2CreERT2 recombination is enriched in Type 1 hair cells in the mature maculae and cristae of the vestibular system. The mammalian inner ear contains six sensory patches that allow detection of auditory stimuli as well as movement and balance. Much research has focused on the organ of Corti, the sensory organ of the cochlea that detects sound. Unfortunately, these cells are difficult to access in vivo , especially in the mature animal, but the development of genetically modified mouse models, including Cre/Lox mice, has improved the ability to label, purify or manipulate these cells. Here, we describe a new tamoxifen-inducible CreER mouse line, the Fbxo2CreERT2 mouse, that can be used to specifically manipulate cells throughout the cochlear duct of the neonatal and mature cochlear epithelium. In vestibular sensory epithelia, Fbxo2CreERT2 -mediated recombination occurs in many hair cells and more rarely in supporting cells of neonatal and adult mice, with a higher rate of Fbxo2CreERT2 induction in type 1 versus type 2 hair cells in adults. Fbxo2CreERT2 mice, therefore, are a new tool for the specific manipulation of epithelial cells of the inner ear and targeted manipulation of vestibular type 1 hair cells. [ABSTRACT FROM AUTHOR]