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A simplified method for detecting Gram-positive and Gram-negative bacteria in dental histological samples: A preliminary and comparative study.
- Source :
-
Acta Histochemica . Jan2023, Vol. 125 Issue 1, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Bacteria identification inside the dental tissue is a complex procedure requiring specific protocols. This study aimed to compare two classical Gram staining methods with a new staining method proposed by the authors to detect Gram-positive and Gram-negative bacteria in dental histological samples of human dentin. Ten human teeth, extracted because of various pathologies, were decalcified, dehydrated, and paraffin-embedded. Then, approximately 100 serial sections of 4 µm thickness were made per sample. The serial sections were placed on glass slides and were stained according to Brown-Brenn, Brown-Hopps, and a proposed modification of Brown-Brenn staining. Both ATCC strains, smeared on glass slides, were stained following each method's instructions used in histological samples. From a qualitative evaluation, the Brown-Brenn method resulted in better staining of Gram-positive bacteria, while the authors' proposed staining technique was more oriented towards Gram-negative bacteria. On the other hand, the Brown-Hopps showed quite a balance in detecting Gram-positive and Gram-negative bacteria. Unlike the Brown-Brenn stain, the other two protocols showed better stainability of Gram-negative microorganisms in bacterial-smeared samples. All staining techniques evaluated in this article can identify bacteria, but the outcome can change according to the staining procedure used. • G&S stain by changing the differentiation method could enhance Gram – bacteria identification in paraffin embeddend samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00651281
- Volume :
- 125
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Acta Histochemica
- Publication Type :
- Academic Journal
- Accession number :
- 161400337
- Full Text :
- https://doi.org/10.1016/j.acthis.2022.151992