Microfluidic bioanalysis based on nanozymes.

As a powerful tool, microfluidics has been widely used in bioanalysis due to the advantages of high portability, fewer sample requirements and rapid detection time. To achieve selective and sensitive detection, enzymes are commonly used as biorecognition elements. However, enzymes used in bioanalysis still suffer from high cost and low stability. Therefore, emerging nanozymes (nanomaterials with enzyme-like characteristics) have been developed to replace enzymes to address their shortcomings. The microfluidic devices based on nanozymes have attracted a lot of interest and shown great promise in bioanalysis via a simple, rapid, and portable approach. This review focuses on recent advances in typical nanozymes and nanozymes involved in cascaded reactions used in microfluidic bioanalysis. Additionally, the future challenges and perspectives are discussed for further development. [Display omitted] • Diverse enzyme-like activities of nanozymes were particularly highlighted. • Microfluidic bioanalyses using individual nanozymes were summarized. • Microfluidic bioanalyses based on nanozymes involved in cascade reactions were summarized. • Future trends in microfluidic bioanalysis based on nanozymes were proposed. [ABSTRACT FROM AUTHOR]