Glucosamine preparation from waste of shrimp processing and its cytotoxic activity against murine leukemia P-388 cell.

Glucosamine has been prepared from shrimp processing waste. Shrimp processing waste is part of the non-meat organ consisting of the skin, head, antennae, and tail. This waste is obtained from the shrimp processing industry PT Ujung Timur in Sidoarjo, East Java - Indonesia. The preparation was carried out in four stages, namely (1) deproteination, (2) demineralization, (3) deacetylation, and (4) hydrolysis. The first stage (deproteination) aims to remove the protein contained in shrimp waste. The second stage (demineralization) aims to remove calcium carbonate and other minerals to produce chitin. Thus, this stage is to isolate chitin (poly N-acetyl glucosamine) from shrimp waste. The third stage (deacetylation) is to remove the acetyl group in chitin to produce chitosan. The fourth stage (hydrolysis) is to hydrolyze chitosan (poly glucosamine) into its monomer, namely glucosamine (2-amino-2-deoxy-D-glucose). The chitin yield was 31.25% by weight of the waste, and the glucosamine yield was 10.15%. Glucosamine preparation results are solid, yellowish-white (white bone) colored, with a melting point of 152-1550C. The compound is soluble in water, slightly soluble in acetone, and insoluble in chloroform. The glucosamine preparation results did not show its cytotoxic activity against P-388 murine leukemia cells. [ABSTRACT FROM AUTHOR]