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Involvement of caspase-3 in apoptosis of human lymphocytes exposed to cadmium chloride.

Authors :
Mirkamali, Mona
Momeni, Hamid Reza
Etemadi, Tahereh
Mosayebi, Ghasem
Komijani, Majid
Source :
Human & Experimental Toxicology. Jan-Dec2022, Vol. 41, p1-9. 9p.
Publication Year :
2022

Abstract

Background: Lymphocytes are a group of white blood cells with a variety of roles their integrity is crucial for the body's immune responses. Cadmium, a heavy metal and environmental pollutant, is known as a toxicant to exert its adverse effects on some sort of cells including blood cells. Research Design: In this study, human lymphocytes were divided into 3 groups: (1) lymphocytes at 0-h, (2) lymphocytes at 24 h (control), (3) lymphocytes treated with cadmium chloride (15 μM). Lymphocyte viability and plasma membrane integrity were assessed in these groups. In addition, the occurrence of apoptosis was investigated by assessment of nucleus diameter and flow cytometry. Activation of caspase-3 was also detected by immunocytochemistry. Results: Result showed that lymphocyte's viability and plasma membrane integrity decreased in lymphocytes treated with cadmium as compared with the control group. Decreased nucleus diameter and result of flow cytometry demonstrated cadmium-induced apoptosis in human lymphocytes. Furthermore, lymphocytes treated with cadmium displayed intensely activated caspase-3 immunoreactivity in their cytoplasm. Conclusion: In conclusion, cadmium not only negatively effect on viability and plasma membrane, but also induces caspase-dependent apoptosis in human lymphocytes. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09603271
Volume :
41
Database :
Academic Search Index
Journal :
Human & Experimental Toxicology
Publication Type :
Academic Journal
Accession number :
161487129
Full Text :
https://doi.org/10.1177/09603271221121796