Bacterial consortium (Priestia endophytica NDAS01F, Bacillus licheniformis NDSA24R, and Priestia flexa NDAS28R) and thiourea mediated amelioration of arsenic stress and growth improvement of Oryza sativa L.

The present study analyzed the effects of individual microbes and their consortium (Priestia endophytica NDAS01F , Bacillus licheniformis NDSA24R, and P. flexa NDAS28R) either alone or in interaction with thiourea (TU) on growth and responses of rice plants subjected to As stress (50 mg kg−1 in soil) in a pot experiment. The bacteria used in the experiment were isolated from As contaminated fields of Nadia, West Bengal and showed significant As removal potential in in vitro experiment. The results revealed significant growth improvement, biomass accumulation, and decline in malondialdehyde levels in rice plants in bacterial and TU treatments as compared to control As treatment. The best results were observed in a bacterial consortium (B1-2-3), which induced a profound increase of 65%, 43%, 127% and 83% in root length, shoot length, leaf width and fresh weight, respectively. Sulfur metabolism and cell wall synthesis were stimulated upon bacterial and TU amendment in plants. The maximum reduction in As concentration was observed in B2 in roots (−55%) and in B1-2-3 in shoot (−83%). The combined treatment of B1-2-3 + TU proved to be less effective as compared to that of B1-2-3 in terms of As reduction and growth improvement. Hence, the usage of bacterial consortium obtained in the present work is a sustainable approach, which might find relevance in field conditions to achieve As reduction in rice grains and to attain higher growth of plants without the need for additional TU supplementation. [Display omitted] • Bacteria isolated from Nadia, West Bengal were used for consortium. • Bacterial consortium promoted growth of plants and reduced arsenic concentration. • Arsenic translocation from root to shoot was significantly decreased. • Bacterial consortium + thiourea did not result in synergistic effects on growth. • Bacterial consortium + thiourea did not cause additional arsenic reduction. [ABSTRACT FROM AUTHOR]