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Advances in CRISPR-Cas9 for the Baculovirus Vector System: A Systematic Review.

Authors :
Sari-Ak, Duygu
Alomari, Omar
Shomali, Raghad Al
Lim, Jackwee
Govinda Raj, Deepak B. Thimiri
Source :
Viruses (1999-4915). Jan2023, Vol. 15 Issue 1, p54. 17p.
Publication Year :
2023

Abstract

The baculovirus expression vector systems (BEVS) have been widely used for the recombinant production of proteins in insect cells and with high insert capacity. However, baculovirus does not replicate in mammalian cells; thus, the BacMam system, a heterogenous expression system that can infect certain mammalian cells, was developed. Since then, the BacMam system has enabled transgene expression via mammalian-specific promoters in human cells, and later, the MultiBacMam system enabled multi-protein expression in mammalian cells. In this review, we will cover the continual development of the BEVS in combination with CRPISPR-Cas technologies to drive genome-editing in mammalian cells. Additionally, we highlight the use of CRISPR-Cas in glycoengineering to potentially produce a new class of glycoprotein medicines in insect cells. Moreover, we anticipate CRISPR-Cas9 to play a crucial role in the development of protein expression systems, gene therapy, and advancing genome engineering applications in the future. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19994915
Volume :
15
Issue :
1
Database :
Academic Search Index
Journal :
Viruses (1999-4915)
Publication Type :
Academic Journal
Accession number :
161560506
Full Text :
https://doi.org/10.3390/v15010054