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Long-term manure amendment reduces nitrous oxide emissions through decreasing the abundance ratio of amoA and nosZ genes in an Ultisol.
- Source :
-
Applied Soil Ecology . Apr2023, Vol. 184, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Organic fertilizers are frequently applied to soils to improve soil multifunctionality and maintain crop productivity in agricultural practices. However, how organic fertilizations influence soil nitrous oxide (N 2 O) emissions remains ambiguous, especially considering their linkage with the abundance of nitrogen (N)-cycling microorganisms. Here, we conducted an incubation experiment to examine the effect of partial replacement of mineral fertilizers with organic fertilizers on the abundance of N-cycling functional genes and N 2 O emissions at 60 % and 100 % water-filled pore space (WFPS) in an acidic Ultisol. Soil samples were collected from 32-year fertilization trials, including four treatments of control (no fertilizer), mineral NPK fertilizer (NPK), NPK + Peanut Straw (NPKS), and NPK + Pig Manure (NPKM). The N 2 O emissions from soils incubated at 100 % WFPS were significantly higher than those at 60 % WFPS in all treatments. Compared with NPK, NPKS and NPKM reduced N 2 O emissions, with a more pronounced effect in NPKM. The N 2 O flux was positively associated with the abundance of AOA and AOB amoA genes, and the abundance ratio of amoA / nosZ (i.e., the ratio of AOA amoA plus AOB amoA and nosZ І plus nosZ ІІ) when the peak N 2 O flux appeared. Random forest analysis showed that the abundance ratio of amoA / nosZ was the best predictor for N 2 O emissions. Compared with NPK, NPKM increased soil pH but reduced the abundance ratio of amoA / nosZ , which might be the key factor in reducing N 2 O emissions. Together, our findings suggested that long-term manure application reduced N 2 O emissions by decreasing the abundance ratio of amoA / nosZ and provided novel insights into the impact of organic fertilizers on N 2 O emissions in acidic agricultural soils. • N 2 O emissions are higher in soils incubated at 100 % WFPS than at 60 % WFPS. • Partial replacement of mineral fertilizers with pig manure reduces soil N 2 O emissions. • N 2 O emissions are positively associated with the abundance ratio of amoA / nosZ. • Manure application increases soil pH and reduces the abundance ratio of amoA / nosZ. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09291393
- Volume :
- 184
- Database :
- Academic Search Index
- Journal :
- Applied Soil Ecology
- Publication Type :
- Academic Journal
- Accession number :
- 161627674
- Full Text :
- https://doi.org/10.1016/j.apsoil.2022.104771