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The polymorphic analysis of cox1 and cob genes of Echinococcus granulosus in the Ngari region of Tibet in China.
- Source :
-
Acta Tropica . Mar2023, Vol. 239, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Cystic echinococcosis (CE) is an important zoonotic parasitic disease caused by Echinococcus granulosus (E. granulosus). CE seriously threatens human health and the development of animal husbandry. The Ngari region is one of the world's highest endemic regions for CE, while genetic polymorphisms of E. granulosus were unclear. Paraffin slices of liver Cyst were collected from seventy-nine surgical patients with echinococcosis in the Ngari region. DNA was extracted from samples. The cox1 and cob genes of mitochondrial DNA of E. granulosus were simultaneously amplified and sequenced. The sequencing results were compared with the standard sequence (KU925397.1and HF947574.1). Phylogenetic trees and the haplotype network of cob and cox 1 genes were constructed and analyzed genotypes of E. granulosus isolated from humans in the Ngari Region of Tibet. Out of 79 hydatid cyst samples collected from surgery patients, 60 isolates were identified as G1/ G3, and two isolates were identified as G6/ G7. Analysis of the cob / cox 1 genes revealed 9/7 mutations resulting in 8/6 haplotypes, respectively. The cob and cox 1 neutrality indices computed by Tajima's D and Fu's Fs tests showed high negative values in Echinococcus granulosus sensu stricto (E. granulosus s. s.). The result suggested that E. granulosus in the Ngari region experienced population expansion or a negative selection. We found that G1/ G3 was still the main genotype, and G6/ G7 was found occasionally in humans of the Ngari region. Therefore, we recommend future surveys and control efforts to investigate G1/ G3 and G6/ G7 transmission in the Ngari region. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0001706X
- Volume :
- 239
- Database :
- Academic Search Index
- Journal :
- Acta Tropica
- Publication Type :
- Academic Journal
- Accession number :
- 161661186
- Full Text :
- https://doi.org/10.1016/j.actatropica.2022.106803