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Highly efficient pretreatment at low enzyme loading from reed: Synergy of liquid hot water–ammonia/oxygen and non-ionic surfactants as enzymatic hydrolysis additive.
- Source :
-
Industrial Crops & Products . Mar2023, Vol. 193, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- This study aimed to obtain a pretreatment and enzymatic hydrolysis method with high enzymatic digestibility at low enzyme loading from reed. Meanwhile, the three major components of reed were separated to be high-value utilized. The combination of liquid hot water–ammonia/oxygen pretreatment and non-ionic surfactants-enhanced enzymatic hydrolysis was studied to solve the problem of non-separation of hemicellulose and lignin in the pretreatment of single-step ammonia/oxygen method. The promoting effect and mechanism of non-ionic surfactants on enzymatic hydrolysis were also explored. Under the optimal conditions, 86.74% of glucan was retained and 65.48% of lignin was dissolved for high-value utilization. The addition of polyethylene glycol increased the enzymatic digestibility of pretreated reed to 97.13% at 9 filter paper units/g–solid. Therefore, this LHW–NH 3 ·H 2 O/O 2 pretreatment and enzymatic hydrolysis adding PEG 6000 method is a low-cost, green, and highly efficient method for fermentable sugar, hemicellulose, and modified lignin production. [Display omitted] • LHW–NH 3 ·H 2 O/O 2 pretreatment is a low-cost, green, and highly efficient method. • Enzymatic digestibility could reach to 97.13%, and 83.11% of glucan was retained. • Low enzyme loading (9 FPU/g–PSs) was used and 40% of cellulase can be saved. • The three components of reed were effectively separated to be high-value utilized. • PEG 6000 can maintain effective enzyme concentration and cellulase activity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09266690
- Volume :
- 193
- Database :
- Academic Search Index
- Journal :
- Industrial Crops & Products
- Publication Type :
- Academic Journal
- Accession number :
- 161661498
- Full Text :
- https://doi.org/10.1016/j.indcrop.2023.116257