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Hsa_circ_0009128 mediates progression of oral squamous cell carcinoma by influencing MMP9.
- Source :
-
Oral Diseases . Mar2023, Vol. 29 Issue 2, p661-671. 11p. 1 Color Photograph, 1 Black and White Photograph, 1 Chart, 3 Graphs. - Publication Year :
- 2023
-
Abstract
- Objectives: The objective of this study was to explore the role and mechanism of an abnormally expressed circRNA (hsa_circ_0009128) in the progression of oral squamous cell carcinoma. Materials and Methods: We conducted quantitative real‐time PCR (qRT‐PCR) to assess the expression of hsa_circ_0009128 in 51 paired OSCC tissues and analyzed the correlation between clinical features and aberrant expression of hsa_circ_0009128 in OSCC. CCK‐8 assays, colony formation assays, transwell assays, and wound healing assays were used to analyze the effect of hsa_circ_0009128 on cell proliferation and migration. Furthermore, epithelial–mesenchymal transition (EMT)‐related proteins were evaluated by Western blotting. Results: Hsa_circ_0009128 was upregulated in both OSCC tissues and cell lines, and high hsa_circ_0009128 correlated with advanced TNM stage (p = 0.046) and lymph node metastasis (p = 0.018) in OSCC patients. Knockdown of hsa_circ_0009128 inhibited cell proliferation and migration. Mechanistically, hsa_circ_0009128 downregulation decreased EMT in OSCC cells as shown by elevated levels of E‐cadherin and decreased N‐cadherin, vimentin, and MMP9. Conclusions: The circRNA hsa_circ_0009128 correlates with malignant progression of OSCC. Hsa_circ_0009128 stimulates proliferation and migration in OSCC cells by targeting MMP9 to activate EMT. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CIRCULAR RNA
*DISEASE progression
*BIOMARKERS
*REVERSE transcriptase polymerase chain reaction
*WOUND healing
*MOUTH tumors
*CELL migration
*MICROBIOLOGICAL assay
*WESTERN immunoblotting
*QUANTITATIVE research
*METASTASIS
*EPITHELIAL-mesenchymal transition
*GENE expression profiling
*CELL proliferation
*RESEARCH funding
*CELL lines
*SQUAMOUS cell carcinoma
Subjects
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 29
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 161690513
- Full Text :
- https://doi.org/10.1111/odi.14019