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RT-RPA-Cas12a-based assay facilitates the discrimination of SARS-CoV-2 variants of concern.
- Source :
-
Sensors & Actuators B: Chemical . Apr2023, Vol. 381, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Timely and accurate detection of SARS-CoV-2 variants of concern (VOCs) is urgently needed for pandemic surveillance and control. Great efforts have been made from a mass of scientists in increasing the detection sensitivity and operability, and reducing the turn-around time and cost. Here, we report a nucleic acid testing-based method aiming to detect and discriminate SARS-CoV-2 mutations by combining RT-RPA and CRISPR-Cas12a detecting assays (RRCd). With a detection limit of 10 copies RNA/reaction, RRCd was validated in 194 clinical samples, showing 89% positive predictive agreement and 100% negative predictive agreement, respectively. Critically, using specific crRNAs, representatives of single nucleotide polymorphisms and small deletions in SARS-CoV-2 VOCs including N501Y, T478K and ΔH69-V70 were discriminated by RRCd, demonstrating 100% specificity in clinical samples with C t < 33. The method completes within 65 min and could offer visible results without using any electrical devices, which probably facilitate point-of-care testing of SARS-CoV-2 variants and other epidemic viruses. • A SARS-CoV-2 detection method (RRCd) was developed. • RRCd can detect and discriminate SARS-CoV-2 mutations. • RRCd was validated in 194 clinical samples showing high accuracy and sensitivity. • RRCd is independent of bulk and electrical devices. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 381
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 161939484
- Full Text :
- https://doi.org/10.1016/j.snb.2023.133433