推拿退热六法对脂多糖致热幼兔下丘脑COX-2 / PGE2 / EP3 信号通路的影响.

Objective 　To observe the antipyretic effect of Tuina antipyretic six methods on lipopolysaccharide (LPS)-induced fever in infant rabbits, and to explore the effects on the hypothalamic COX-2/PGE2/EP3 signaling pathway. Methods 　Using the random number table method, 24 New Zealand rabbits(two months old)were randomly divided into the control group, model group and Tuina six methods group, with eight rabbits per group. The fever model was replicated by LPS (0. 5 mg/L) injection at the ear margin, and the injection volume was 1 mL/kg in model group and Tuina six methods group. In the Tuina six methods group, the following Tuina six methods of antipyretic intervention were used 1 h after LPS injection: opening Tianmen 200 times in 2 min, pushing Kangong 200 times in 2 min, rubbing Taiyang 200 times in 2 min, rubbing Erhougaogu 24 times and pinching three times in 2 min, clearing the Tianheshui 500 times in 5 min, and pushing the spine 300 times in 5 min. Over the same period, the animals in the model and control groups were smeared with the same volume room temperature water in the same are, and grasped without Tuina intervention. The anal temperature was recorded once every 20 min after LPS injection, and the temperature was continuously measured for 180 min. The body temperature of each group was observed, and the maximum body temperature change value and thermal response index (TRI) were calculated. The expression levels of PGE2 and cAMP in the hypothalamus were detected by ELISA, the protein expression levels of COX-2, EP3, and cAMP were detected using Western blotting, and the mRNA expression levels of COX-2, EP3, and cAMP were detected using RT-PCR. Results　Compared with the control group, the anal temperature of the model group was increased (P<0. 01), and the anal temperature of the model infant rabbits in the Tuina six methods group was decreased after the intervention (P<0. 01). The maximum body temperature change value and the TRI in the model group and the Tuina six methods group were higher than those in the control group at 60 min after modeling (P <0. 01). Compared with the model group, there was no difference in the maximum body temperature change value and TRI 60 min after modeling in the Tuina six methods group. Moreover, 180 min after modeling, the maximum body temperature change value and TRI were increased in the model group(P<0. 01), and those were decreased in the Tuina six methods group compared with those in the model group (P < 0. 01). The ELISA result showed that compared with the control group, the expression levels of PGE2 and cAMP in the hypothalamus of the model group were increased (P<0. 01), while those in the Tuina six methods group were lower than those in the model group (P<0. 05, P<0. 01). The result of Western blotting showed that compared with the control group, the expression levels of COX-2, EP3, and cAMP in the hypothalamus of the model group were increased (P<0. 01), while those in the Tuina six methods group were lower than those in the model group (P<0. 05, P<0. 01). The RT-PCR result showed that compared with the control group,the mRNA expression levels of COX-2, EP3, and cAMP in the hypothalamus of the model group were increased (P <0. 01), and those in the Tuina six methods group were decreased, compared with those in the model group (P<0. 01). Conclusion　Tuina antipyretic six methods shows a good antipyretic effect on LPS-induced fever infant rabbits. Its mechanism is achieved by inhibiting the activation of the hypothalamic COX-2/PGE2/EP3 signaling pathway by pyrogen, reducing the level of positive regulatory medium cAMP at the terminal of the body temperature center. [ABSTRACT FROM AUTHOR]