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Cyclooxygenase-2 activates EGFR–ERK1/2 pathway via PGE2-mediated ADAM-17 signaling in testosterone-induced benign prostatic hyperplasia.

Authors :
Abo-El Fetoh, Mohammed E.
Abdel-Fattah, Maha M.
Mohamed, Wafaa R.
Ramadan, Laila A. A.
Afify, Hassan
Source :
Inflammopharmacology. Feb2023, Vol. 31 Issue 1, p499-516. 18p.
Publication Year :
2023

Abstract

Objective and design: Prostatic inflammation is the driving force in benign prostatic hyperplasia (BPH). This work investigated the potential modulatory effect of COX-2 inhibition on ADAM-17/EGFR/ERK1/2 axis. Materials or subjects: Adult male Wistar rats were used. Treatment: Celecoxib (10 and 20 mg/kg; i.p.) was injected i.p. daily for three weeks. Testosterone (TST) (3 mg/kg; s.c.) was used to induce BPH. Methods: Prostatic inflammation and hyperplasia were assessed by organ weight and histopathology. Inflammatory mediators were measured using ELISA technique. Protein analysis was performed using western blotting and immunohistochemistry. Gene expression analysis was performed using qRT-PCR. Statistical analyses included one-way ANOVA and Tukey's multiple comparison test. Results: Testosterone-treated rats had a marked increase in COX-2, prostate weight, and index. Moreover, TST-induced COX-2 was inferred from cytoskeletal changes and was attributable to the overexpression of PGE2, NF-κB (p65), and IL-6. COX-2-derived PGE2 increased the activity of ADAM-17, TGF-α, and TNF-α. Consequently, EGFR–ERK1/2 pathway was over-activated, disrupting anti-apoptotic Bcl-2, cyclin D1, and pro-apoptotic Bax. Celecoxib reversed these effects. Conclusion: COX-2 stimulates the ERK1/2 pathway via PGE2–ADAM-17-catalyzed shedding of TGF-α in testosterone-induced BPH. The results indicate a functional correlation between inflammation and hyperplasia in BPH. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09254692
Volume :
31
Issue :
1
Database :
Academic Search Index
Journal :
Inflammopharmacology
Publication Type :
Academic Journal
Accession number :
162076965
Full Text :
https://doi.org/10.1007/s10787-022-01123-7