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A long-wavelength mitochondria-targeted fluorescent probe for imaging of peroxynitrite during dexamethasone treatment.
- Source :
-
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy . May2023, Vol. 292, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- [Display omitted] • Probe is capable of detecting ONOO− with a longwave fluorescence emission. • TL could monitor high concentration of dexamethasone-induced an up-regulation of ONOO−. • Probe has mitochondrial targeting capability. • Probe have good selectivity and low detection limit. • The imaging demonstrated its value of practical application. Peroxynitrite (ONOO−), as a strong oxidizing reactive nitrogen substance (RNS), is generated endogenously by cells. Its visualization research is crucial to understand relevant disease processes. Herein, we reported a long-wavelength mitochondria-targeted fluorescence "turn on" probe TL. The probe TL could react with ONOO− by using 4-(Bromomethyl)benzeneboronic as a reactive site, which exhibited outstanding characteristics for detection of ONOO−, thus improving response time (about 50 s), sensitivity (DL, 10.1 nM), and emission wavelength (667 nm). Besides, TL displayed well mitochondria targeting and biological visualizing of exogenous and endogenous ONOO− in biological systems. Finally, TL was used to monitor high concentration of dexamethasone-induced an up-regulation of ONOO−. This indicated that TL has excellent potential to study the fluctuation of ONOO− in the physiological and pathological system. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13861425
- Volume :
- 292
- Database :
- Academic Search Index
- Journal :
- Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 162176484
- Full Text :
- https://doi.org/10.1016/j.saa.2023.122429