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CRISPR-based nucleic acid diagnostics for pathogens.
- Source :
-
Trends in Analytical Chemistry: TRAC . Mar2023, Vol. 160, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Pathogenic infection remains the primary threat to human health, such as the global COVID-19 pandemic. It is important to develop rapid, sensitive and multiplexed tools for detecting pathogens and their mutated variants, particularly the tailor-made strategies for point-of-care diagnosis allowing for use in resource-constrained settings. The rapidly evolving CRISPR/Cas systems have provided a powerful toolbox for pathogenic diagnostics via nucleic acid tests. In this review, we firstly describe the resultant promising class 2 (single, multidomain effector) and recently explored class 1 (multisubunit effector complexes) CRISPR tools. We present diverse engineering nucleic acid diagnostics based on CRISPR/Cas systems for pathogenic viruses, bacteria and fungi, and highlight the application for detecting viral variants and drug-resistant bacteria enabled by CRISPR-based mutation profiling. Finally, we discuss the challenges involved in on-site diagnostic assays and present emerging CRISPR systems and CRISPR cascade that potentially enable multiplexed and preamplification-free pathogenic diagnostics. • Class 2 and recently explored class 1 CRISPR tools are presented. • POCT strategies for detecting pathogenic viruses, bacteria and fungi are introduced. • CRISPR-based profiling of viral variants and drug-resistant bacteria is highlighted. • Challenges for multiplexing and preamplification-free diagnostic assay are discussed. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01659936
- Volume :
- 160
- Database :
- Academic Search Index
- Journal :
- Trends in Analytical Chemistry: TRAC
- Publication Type :
- Academic Journal
- Accession number :
- 162254144
- Full Text :
- https://doi.org/10.1016/j.trac.2023.116980