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Metabolic engineering of Escherichia coli for the production of an antifouling agent zosteric acid.
- Source :
-
Metabolic Engineering . Mar2023, Vol. 76, p247-259. 13p. - Publication Year :
- 2023
-
Abstract
- Zosteric acid (ZA) is a Zostera species-derived, sulfated phenolic acid compound with antifouling activity and has gained much attention due to its nontoxic and biodegradable characteristics. However, the yield of Zostera species available for ZA extraction is limited by natural factors, such as season, latitude, light, and temperature. Here we report the development of metabolically engineered Escherichia coli strains capable of producing ZA from glucose and glycerol. First, intracellular availability of the sulfur donor 3′-phosphoadenosine-5′-phosphosulfate (PAPS) was enhanced by knocking out the cysH gene responsible for PAPS consumption and overexpressing the genes required for PAPS biosynthesis. Co-overexpression of the genes encoding tyrosine ammonia-lyase, sulfotransferase 1A1, ATP sulfurylase, and adenosine 5′-phosphosulfate kinase constructed ZA producing strain with enhanced PAPS supply. Second, the feedback-resistant forms of aroG and tyrA genes (encoding 3-deoxy- d -arabinoheptulosonate 7-phosphate synthase and chorismate mutase, respectively) were overexpressed to relieve the feedback regulation of L- tyrosine biosynthesis. Third, the pykA gene involved in phosphoenolpyruvate-consuming reaction, the regulator gene tyrR , the competing pathway gene pheA , and the ptsHIcrr genes essential for the PEP:carbohydrate phosphotransferase system were deleted. Moreover, all genes involved in the shikimate pathway and the talA , tktA , and tktB genes in the pentose phosphate pathway were examined for ZA production. The PTS-independent glucose uptake system, the expression vector system, and the carbon source were also optimized. As a result, the best-performing strain successfully produced 1.52 g L−1 ZA and 1.30 g L−1 p -hydroxycinnamic acid from glucose and glycerol in a 700 mL fed-batch bioreactor. • Multi-level metabolic engineering of E. coli allowed p -hydroxycinnamic acid and zosteric acid production. • Knockout of cysH and overexpression of ATPSL and APSK synergically improved intracellular PAPS content. • Accumulation of p -hydroxycinnamic acid hindered sulfation in zosteric acid biosynthesis. • Fed-batch culture produced 1.52 g L−1 zosteric acid and 1.30 g L−1 p -hydroxycinnamic acid. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10967176
- Volume :
- 76
- Database :
- Academic Search Index
- Journal :
- Metabolic Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 162288885
- Full Text :
- https://doi.org/10.1016/j.ymben.2023.02.007