Back to Search Start Over

Lamin A/C phosphorylation at serine 22 is a conserved heat shock response to regulate nuclear adaptation during stress.

Authors :
Virtanen, Laura
Holm, Emilia
Halme, Mona
West, Gun
Lindholm, Fanny
Gullmets, Josef
Irjala, Juho
Heliö, Tiina
Padzik, Artur
Meinander, Annika
Eriksson, John E.
Taimen, Pekka
Source :
Journal of Cell Science. Feb2023, Vol. 136 Issue 4, p1-14. 14p.
Publication Year :
2023

Abstract

The heat shock (HS) response is crucial for cell survival in harmful environments. Nuclear lamin A/C, encoded by the LMNA gene, contributes towards altered gene expression during HS, but the underlying mechanisms are poorly understood. Here, we show that upon HS, lamin A/C was reversibly phosphorylated at serine 22 in concert with HSF1 activation in human cells, mouse cells and Drosophila melanogaster in vivo. Consequently, the phosphorylation facilitated nucleoplasmic localization of lamin A/C and nuclear sphericity in response to HS. Interestingly, lamin A/C knock-out cells showed deformed nuclei after HS and were rescued by ectopic expression of wild-type lamin A, but not by a phosphomimetic (S22D) lamin A mutant. Furthermore, HS triggered concurrent downregulation of lamina-associated protein 2α (Lap2α, encoded by TMPO) in wild-type lamin A/C-expressing cells, but a similar response was perturbed in lamin A/C knock-out cells and in LMNA mutant patient fibroblasts, which showed impaired cell cycle arrest under HS and compromised survival at recovery. Taken together, our results suggest that the altered phosphorylation stoichiometry of lamin A/C provides an evolutionarily conserved mechanism to regulate lamina structure and serve nuclear adaptation and cell survival during HS. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219533
Volume :
136
Issue :
4
Database :
Academic Search Index
Journal :
Journal of Cell Science
Publication Type :
Academic Journal
Accession number :
162410918
Full Text :
https://doi.org/10.1242/jcs.259788