Historical biogeography of Cannabis in the Iberian Peninsula: A probabilistic approach using palynological evidence.

The tempo and mode of colonization of the Iberian Peninsula (IP) by Cannabis sativa , its further internal spreading and the potential cultural and environmental factors involved remain unknown. The available continental-wide European meta-analyses using pollen and archeological evidence account for only a few IP sites, insufficient for a sound assessment. This paper presents a nearly comprehensive database of almost 60 IP sites with palynological evidence of Cannabis and analyzes the corresponding spatiotemporal patterns. The first scattered records of this pollen type date from the Middle and Upper Paleolithic (150–12 kyr BP) and would have entered the IP by maritime Mediterranean or terrestrial continental pathways, or both. A first burst of introductions, probably in a cultivated form, would have occurred during the Neolithic (7–5 kyr BP) using similar paths. Human participation in these Neolithic introductions remains unclear but cannot be dismissed. A period of reduced Cannabis arrivals (mostly via maritime pathway) occurred between the Chalcolithic and the Roman Epoch (4.5–2 kyr BP), when the innermost parts of the IP were colonized (Late Bronze). A second, likely anthropogenic, introduction acceleration took place in the Middle Ages (1.5 kyr BP onward) using the Mediterranean and the continental pathways. Maximum cultivation and hemp retting activity was recorded during the Modern Ages (16th-19th centuries), coinciding with the increased demand of hemp fiber to supply the Spanish royal navy for imperial expansion and commerce. A potential link between Cannabis colonization/introduction bursts and climatic warmings has been observed that should be tested with future studies. Regional moisture variations seem to be less influential. Further efforts to enhance and improve the database used in this study are encouraged. The results of this paper should be compared with archeological and historical evidence to clarify the role of human migrations and cultural changes in the historical biogeography of Cannabis in the IP. • Cannabis entered the Iberian Peninsula (IP) as soon as ∼150 kyr BP likely by wild dispersal. • The first burst of introductions occurred in the Neolithic (7–5 kyr BP). • Spreading across the whole IP occurred the Chalcolithic-Roman periods (4.5–2 kyr BP). • An introduction acceleration took place in the Middle Age (1.5 kyr BP onward). • Maximum hemp cultivation/retting occurred in Modern Ages (16th-19th centuries). • Colonization/introduction bursts coincided with warmer climatic phases. [ABSTRACT FROM AUTHOR]