VX2和人骨肉瘤MG⁃63兔成瘤模型的建立和比较.

Objective To investigate the feasibility of transplanting human osteosarcoma MG⁃63 tumor masses into rabbits tumorigenesis model. Methods A total of 24 New Zealand white rabbits were randomly divided into the VX2 group and the MG⁃63 group, with 12 rabbits in each group. VX2 tumor or human osteosarcoma MG⁃63 tumor were implanted into the bone marrow cavity of the right tibia of each rabbit in both groups. Color Doppler ultrasound was performed at 3, 4 and 5 weeks after modeling, and X⁃ray and CT examinations were performed at the end of the 4⁃week ultrasonography to observe the tumorigenic characteristics and tumorigenic rate of the rabbits in both groups. Alkaline phosphatase (AKP) levels were measured before and 4 weeks after modeling. At 3 and 4 weeks after modeling, 3 tumorous rabbits in each group were sacrificed by the air embolization method, and the remaining rabbits were sacrificed at 5 weeks after modeling for pathological examination. Results There were 10 and 11 rabbits surviving in the VX2 and MG⁃63 groups, respectively. The tumor formation rates were 60.0%, 90.0%, 100.0% in the VX2 group and 0, 45.5%, 63.6% in the MG⁃63 group at 1, 2, and 3 weeks after modeling, respectively. The lung metastasis rates were 66.7%, 66.7%, 100.0% in the VX2 group and 0, 33.3%, 40.0%intheMG⁃63groupat3,4,and5weeksaftermodeling,respectively.No abdominal organ metastasis was observed in all the examined rabbits. At 4 weeks after modeling, the serum AKP concentration in the VX2 group was significantly higher than that before modeling and the MG⁃63 group, the differences were statistically significant (P=0.002, <0.001). The results of radiographic and pathological ex⁃ amination showed that significant bone destruction occurred in the VX2 group and slight bone destruction occurred in the MG⁃63 group at 4 weeks after modeling. The rate of bone destruction and tumor bone formation in the VX2 group were higher than those in the MG⁃63 group, but the differences were not statistically significant (P=0.152, 0.072). Conclusions Compared with the rabbit VX2 tumor model, the tumor formation rate and lung metastasis rate of the MG⁃63 rabbit osteosarcoma model are lower, with poor stability. The ideal rabbit osteosarcoma animal model still needs to be further explored. [ABSTRACT FROM AUTHOR]