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Carbon dots-based modularization fluorescent probe for simultaneous detection of Hg2+ and Cu2+ in water, cells and zebrafish.
- Source :
-
Dyes & Pigments . Jun2023, Vol. 214, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Modular assembly of carbon dots with organic luminescent small molecules can be devoted to picking up a predetermined fluorescent probe detection system for specific targets. In this work, the blue fluorescent carbon dots B-CDs were prepared by using sodium citrate and nitrogen-rich branched polyethyleneimine as precursors through a functional preservation strategy. A modularized, difunctional and ratiometric fluorescent probe B-CDs@RBH with dual-emission was dexterously prepared by simply blending B-CDs and rhodamine b-hydrazide for the detection of Hg2+ and Cu2+. B-CDs can be quenched by Hg2+ through static quenching, while Cu2+ can specifically open the lactam of rhodamine b-hydrazide to form rhodamine B through hydrolysis accompanied by a turn-on orange fluorescence. More interestingly, rhodamine B can absorb the fluorescence of B-CDs through fluorescence resonance energy transfer, resulting in a blue to orange fluorescence color change in the presence of Cu2+. B-CDs@RBH has a low detection limit of 17.79 nM and 4.19 nM for Hg2+ and Cu2+ respectively with a detection range of 0.1–5 μM, which was applied for the ratiometric fluorescence detection of Hg2+ and Cu2+ in cells and zebrafish, and achieved smartphone-assisted on-site water samples detection. The facile modular design of B-CDs@RBH fluorescent probe and convenient on-site detection application are of great benefit in further ascertaining the migration and transformation of heavy metals in biological and environmental samples. [Display omitted] • The facile modular fabrication of bifunctional FRET-type fluorescent probe by hybridizing carbon dots and fluorescent dye. • The monitoring of Hg2+ and Cu2+ in water samples, living cells and zebrafish was realized. • Smartphone-assisted on-site quantitative determination of Hg2+ and Cu2+ was realized with no expensive instrument. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01437208
- Volume :
- 214
- Database :
- Academic Search Index
- Journal :
- Dyes & Pigments
- Publication Type :
- Academic Journal
- Accession number :
- 162592656
- Full Text :
- https://doi.org/10.1016/j.dyepig.2023.111232