Back to Search
Start Over
Washing-free electrogenerated chemiluminescence magnetic microbiosensors based on target assistant proximity hybridization for multiple protein biomarkers.
- Source :
-
Analytica Chimica Acta . May2023, Vol. 1253, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- This work reports washing-free electrogenerated chemiluminescence (ECL) magnetic microbiosensors based on target assistant proximity hybridization (TAPH) for multiple protein biomarkers for the first time. As a principle-of-proof, alpha-fetoprotein (AFP) was chosen as a model analyte, and biotin-DNA1 bound streptavidin-coated magnetic microbeads (MMB@SA⋅biotin-DNA1) were designed as the universal capture MMB, while the corresponding two antibodies tagged with DNA2 or DNA3 were utilized as hybrid recognition probes, and ruthenium complex-tagged DNA4-10A was designed as a universal ECL signal probe. When the capture MMB was added into the mixture solution (containing the analyte, hybrid recognition probes, signal probe and tri- n -propylamine), biocomplexes were formed on the MMB. After the resulting MMB was efficiently brought to the surface of a magnetic glassy carbon electrode (MGCE), ECL measurement was performed without a washing step, resulting in an increase in the ECL intensity. A model for ECL measuring the second-order rate constants of hybridization reactions on MMB was derived. It was found that the rate constants for hybridization reactions on MMB in rotating mode are 1.6-fold higher than those in shaking mode, and a suitable DNA length of the signal probe can improve the signal-to-noise ratio. The washing-free ECL method was developed for the determination of AFP with a much lower detection limit (LOD) of 0.04 ng mL−1. The developed flexible strategy has been extended to determine D-dimer with an LOD of 0.1 ng mL−1 and myoglobinglobin with an LOD of 1.1 ng mL−1. This work demonstrated that the proposed strategy of ECL TAPH on MMB at MGCE is a washing-free and flexible promising strategy, and can be extended to qualify other multiple protein biomarkers in real clinical assays. [Display omitted] • TAPH-based ECL assay on magnetic micro-beads exceed to conventional electrode one. • Highly sensitive ECL methods were developed for α-fetoprotein, D-dimer and myoglobin. • Washing-free ECL magnetic biosensors can be constructed with universal signal probe. • Rotating particles can accelerate biological reaction rates on magnetic beads. • Suitable length of universal signal probe can improve the signal to noise ratio. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032670
- Volume :
- 1253
- Database :
- Academic Search Index
- Journal :
- Analytica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 162635539
- Full Text :
- https://doi.org/10.1016/j.aca.2023.340926