Back to Search
Start Over
Enhanced 1,3-propanediol production with high yield from glycerol through a novel Klebsiella–Shewanella co-culture.
- Source :
-
Biotechnology for Biofuels & Bioproducts . 3/24/2023, Vol. 16 Issue 1, p1-9. 9p. - Publication Year :
- 2023
-
Abstract
- Background: 1,3-Propanediol (1,3-PDO) is a platform compound, which has been widely used in food, pharmaceutical and cosmetic industries. Compared with chemical methods, the biological synthesis of 1,3-PDO has shown promising applications owing to its mild conditions and environmental friendliness. However, the biological synthesis of 1,3-PDO still has the problem of low titer and yield due to the shortage of reducing powers. Results: In this study, Klebsiella sp. strain YT7 was successfully isolated, which can synthesize 11.30 g/L of 1,3-PDO from glycerol in flasks. The intracellular redox regulation strategy based on the addition of electron mediators can increase the 1,3-PDO titer to 28.01 g/L. Furthermore, a co-culturing system consisting of strain YT7 and Shewanella oneidensis MR-1 was established, which can eliminate the supplementation of exogenous electron mediators and reduce the by-products accumulation. The 1,3-PDO yield reached 0.44 g/g and the final titer reached 62.90 g/L. The increased titer and yield were attributed to the increased redox levels and the consumption of by-products. Conclusions: A two-bacterium co-culture system with Klebsiella sp. strain YT7 and S. oneidensis strain MR-1 was established, which realized the substitution of exogenous electron mediators and the reduction of by-product accumulation. Results provided theoretical basis for the high titer of 1,3-PDO production with low by-product concentration. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 27313654
- Volume :
- 16
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biotechnology for Biofuels & Bioproducts
- Publication Type :
- Academic Journal
- Accession number :
- 162682623
- Full Text :
- https://doi.org/10.1186/s13068-023-02304-4