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Impact of sub-acute acrolein inhalation on the molecular regulation of mitochondrial metabolism in rat lung.
- Source :
-
Toxicology Letters . Apr2023, Vol. 378, p19-30. 12p. - Publication Year :
- 2023
-
Abstract
- Nowadays, mitochondria are recognized as key players in the pathogenesis of a variety of smoking-associated lung diseases. Acrolein, a component of cigarette smoke, is a known driver of biological mechanisms underlying smoking-induced respiratory toxicity. The impact of sub-acute acrolein inhalation in vivo on key processes controlling mitochondrial homeostasis in cells of the airways however is unknown. In this study, we investigated the activity/abundance of a myriad of molecules critically involved in mitochondrial metabolic pathways and mitochondrial quality control processes (mitochondrial biogenesis and mitophagy) in the lungs of Sprague-Dawley rats that were sub-acutely exposed to filtered air or 3 ppm acrolein by whole-body inhalation (5 h/day, 5 days/week for 4 weeks). Acrolein exposure induced a general inflammatory response in the lung as gene expression analysis revealed an increased expression of Icam1 and Cinc1 (p < 0.1; p < 0.05). Acrolein significantly decreased enzyme activity of hydroxyacyl-Coenzyme A dehydrogenase (p < 0.01), and decreased Pdk4 transcript levels (p < 0.05), suggestive of acrolein-induced changes in metabolic processes. Investigation of constituents of the mitochondrial biogenesis pathways and mitophagy machinery revealed no pronounced alterations. In conclusion, sub-acute inhalation of acrolein did not affect the regulation of mitochondrial metabolism and quality control, which is in contrast to more profound changes after acute exposure in other studies. [Display omitted] • Sub-acute acrolein exposure induces a general inflammatory response in rat lung. • Sub-acute acrolein inhalation does not impact mitochondrial metabolism in lung. • No alterations of mitochondrial quality control indices upon sub-acute acrolein inhalation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *METABOLIC regulation
*HOMEOSTASIS
*ACROLEIN
*METABOLISM
Subjects
Details
- Language :
- English
- ISSN :
- 03784274
- Volume :
- 378
- Database :
- Academic Search Index
- Journal :
- Toxicology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 162704963
- Full Text :
- https://doi.org/10.1016/j.toxlet.2023.02.003