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Dihydroartemisinin Affects STAT3/DDA1 Signaling Pathway and Reverses Breast Cancer Resistance to Cisplatin.
- Source :
-
American Journal of Chinese Medicine . 2023, Vol. 51 Issue 2, p445-459. 15p. - Publication Year :
- 2023
-
Abstract
- Dihydroartemisinin (DHA) has anticancer effects on multiple tumors, including those associated with breast cancer. This study aimed to investigate the mechanism causing DHA-reversing cisplatin (DDP) resistance in breast cancer. Relative mRNA and protein levels were tested using a qRT-PCR and western blot assay. Cell proliferation, viability, and apoptosis were evaluated using colony formation, MTT, and flow cytometry assays, respectively. Interaction of STAT3 and DDA1 was measured via a dual-luciferase reporter assay. The results showed that DDA1 and p-STAT3 levels were dramatically elevated in DDP-resistant cells. DHA treatment repressed proliferation and induced apoptosis of DDP-resistant cells by suppressing STAT3 phosphorylation; the inhibition ability was positively proportional to the DHA concentration. DDA1 knockdown inhibited cyclin expression, promoted G0/G1 phase arrest, restrained cell proliferation, and induced apoptosis of DDP-resistant cells. Furthermore, knockdown of STAT3 restrained proliferation and induced apoptosis and G0/G1 cell cycle arrest of DDP-resistant cells by targeting DDA1. DHA could restrain tumor proliferation of breast cancer via enhancing drug sensitivity of DDP-resistant cells through the STAT3/DDA1 signaling pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEIN analysis
*REVERSE transcriptase polymerase chain reaction
*FLOW cytometry
*WESTERN immunoblotting
*ANTINEOPLASTIC agents
*APOPTOSIS
*CELL cycle proteins
*CELLULAR signal transduction
*CELL survival
*CISPLATIN
*MESSENGER RNA
*CELL proliferation
*BACTERIAL growth
*MICROBIOLOGICAL techniques
*TUMOR markers
*CELL lines
*COLORIMETRY
*DRUG resistance in cancer cells
*BREAST tumors
*PHOSPHORYLATION
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 0192415X
- Volume :
- 51
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- American Journal of Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 162755783
- Full Text :
- https://doi.org/10.1142/S0192415X23500234