Back to Search
Start Over
Conditional protein splicing triggered by SUMO protease.
- Source :
-
Biochemical & Biophysical Research Communications . May2023, Vol. 655, p44-49. 6p. - Publication Year :
- 2023
-
Abstract
- Conditional protein splicing is a powerful biotechnological tool that can be used to post-translationally control the activity of target proteins. Here we demonstrated a novel conditional protein splicing approach in which the small ubiquitin-like modifier (SUMO) protease induced the splicing of an atypical split intein. The engineered Ter DnaE-3 S11 split intein which has a small C-intein segment with only 6 amino acids was used in this study. A SUMO tag was fused to the N-terminus of the C-intein to inhibit the protein trans -splicing in vitro. The splicing products could be detected in 15 min with the addition of SUMO protease by western blotting and the splicing efficiency was ∼4-fold higher than the control without SUMO protease for overnight reaction. This engineered Ter DnaE-3 S11 split intein-mediated protein trans -splicing had been further shown to be triggered by SUMO protease in different exteins in vitro. Our study provides new insights into the regulation of protein splicing and is a promising tool for the control of protein structure and function in vitro. • A novel conditional protein splicing approach was established in vitro. • SUMO protease induced the splicing of an atypical split intein. • SUMO protease triggered conditional protein splicing worked in different exteins. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEIN structure
*PROTEINS
*WESTERN immunoblotting
*AMINO acids
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 655
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 162757746
- Full Text :
- https://doi.org/10.1016/j.bbrc.2023.03.023