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Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes.
- Source :
-
PLoS Biology . 4/3/2023, Vol. 21 Issue 4, p1-33. 33p. 1 Color Photograph, 1 Diagram, 5 Graphs. - Publication Year :
- 2023
-
Abstract
- As the stimulus-responsive mediator of actin dynamics, actin-depolymerizing factor (ADF)/cofilin is subject to tight regulation. It is well known that kinase-mediated phosphorylation inactivates ADF/cofilin. Here, however, we found that the activity of Arabidopsis ADF7 is enhanced by CDPK16-mediated phosphorylation. We found that CDPK16 interacts with ADF7 both in vitro and in vivo, and it enhances ADF7-mediated actin depolymerization and severing in vitro in a calcium-dependent manner. Accordingly, the rate of actin turnover is reduced in cdpk16 pollen and the amount of actin filaments increases significantly at the tip of cdpk16 pollen tubes. CDPK16 phosphorylates ADF7 at Serine128 both in vitro and in vivo, and the phospho-mimetic mutant ADF7S128D has enhanced actin-depolymerizing activity compared to ADF7. Strikingly, we found that failure in the phosphorylation of ADF7 at Ser128 impairs its function in promoting actin turnover in vivo, which suggests that this phospho-regulation mechanism is biologically significant. Thus, we reveal that CDPK16-mediated phosphorylation up-regulates ADF7 to promote actin turnover in pollen. As the stimulus-responsive mediator of actin dynamics, ADF/cofilin is tightly regulated. This study shows that the actin depolymerization and severing activity of Arabidopsis ADF7 is enhanced by CDPK16-mediated phosphorylation at Ser128 to promote actin turnover in pollen. [ABSTRACT FROM AUTHOR]
- Subjects :
- *POLLEN tube
*ACTIN
*PHOSPHORYLATION
*ARABIDOPSIS
*DEPOLYMERIZATION
Subjects
Details
- Language :
- English
- ISSN :
- 15449173
- Volume :
- 21
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- PLoS Biology
- Publication Type :
- Academic Journal
- Accession number :
- 162873540
- Full Text :
- https://doi.org/10.1371/journal.pbio.3002073