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A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy.
- Source :
-
Angewandte Chemie . 4/11/2023, Vol. 135 Issue 16, p1-7. 7p. - Publication Year :
- 2023
-
Abstract
- Exosomal microRNAs (miRNAs) have considerable potential as pivotal biomarkers to monitor cancer development, dis‐ease progression, treatment effects and prognosis. Here, we report an efficient target recycling amplification process (TRAP) for the digital detection of miRNAs using photonic resonator absorption microscopy. We achieve multiplex digital detection with sub‐attomolar sensitivity in 20 minutes, robust selectivity for single nucleotide variants, and a broad dynamic range from 1 aM to 1 pM. Compared with traditional qRT‐PCR, TRAP showed similar accuracy in profiling exosomal miRNAs derived from cancer cells, but also exhibited at least 31‐fold and 61‐fold enhancement in the limits of miRNA‐375 and miRNA‐21 detection, respectively. The TRAP approach is ideal for exosomal or circulating miRNA biomarker quantification, where the miRNAs are present in low concentrations or sample volume, with potentials for frequent, low‐cost, and minimally invasive point‐of‐care testing. [ABSTRACT FROM AUTHOR]
- Subjects :
- *WASTE recycling
*EXOSOMES
*SINGLE nucleotide polymorphisms
*MICRORNA
*RESONATORS
Subjects
Details
- Language :
- English
- ISSN :
- 00448249
- Volume :
- 135
- Issue :
- 16
- Database :
- Academic Search Index
- Journal :
- Angewandte Chemie
- Publication Type :
- Academic Journal
- Accession number :
- 162877892
- Full Text :
- https://doi.org/10.1002/ange.202217932