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Exposure to acrylamide induces zygotic genome activation defects of mouse embryos.
- Source :
-
Food & Chemical Toxicology . May2023, Vol. 175, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
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Abstract
- Acrylamide (ACR) is an important chemical raw material for wastewater treatment, paper industry and textile industry, which is widely exposed from occupational, environmental and dietary situation. ACR has neurotoxicity, genotoxicity, potential carcinogenicity and reproductive toxicity. Recent study indicates that ACR affected oocyte maturation quality. In the present study, we reported the effects of ACR exposure on zygotic genome activation (ZGA) in embryos and its related mechanism. Our results showed that ACR treatment caused 2-cell arrest in mouse embryos, indicating the failure of ZGA, which was confirmed by decreased global transcription levels and aberrant expression of ZGA-related and maternal factors. We found that histone modifications such as H3K9me3, H3K27me3 and H3K27ac levels were altered, and this might be due to the occurrence of DNA damage, showing with positive γ-H2A.X signal. Moreover, mitochondrial dysfunction and high levels of ROS were detected in ACR treated embryos, indicating that ACR induced oxidative stress, and this might further cause abnormal distribution of endoplasmic reticulum, Golgi apparatus and lysosomes. In conclusion, our results indicated that ACR exposure disrupted ZGA by inducing mitochondria-based oxidative stress, which further caused DNA damage, aberrant histone modifications and organelles in mouse embryos. • Acrylamide decreased global transcription levels and ZGA-related gene expression. • Acrylamide altered histone modification levels such as H3K9me3, H3K27me3 and H3K27ac. • Acrylamide caused mitochondria dysfunction and DNA damage. • Acrylamide induced oxidative stress and disrupted organelle functions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ACRYLAMIDE
*GOLGI apparatus
*EMBRYOS
*WASTEWATER treatment
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 02786915
- Volume :
- 175
- Database :
- Academic Search Index
- Journal :
- Food & Chemical Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 162978769
- Full Text :
- https://doi.org/10.1016/j.fct.2023.113753