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Analysis of the Differential Expression and Antiviral Activity of Porcine Interferon-α In Vitro.
- Source :
-
International Journal of Peptide Research & Therapeutics . May2023, Vol. 29 Issue 3, p1-11. 11p. - Publication Year :
- 2023
-
Abstract
- Porcine interferon α (poIFN-α) is a crucial cytokine that can prevent and treat viral infections. Seventeen functional porcine IFN-α subtypes were found in the porcine genome. In this study, multiple sequence alignment was performed to analyze IFN-α protein structure and function. Phylogenetic tree analysis of the poIFN gene family defined the evolutionary relationship of various subtypes. PoIFN-αs, including poIFN-α1–17, were expressed in an Escherichia coli expression system. The antiviral activities of these IFN-α proteins against vesicular stomatitis virus (VSV) and pseudorabies virus (PRV) were examined in PK-15 cells. We found that the antiviral activity of different poIFN-α molecules greatly differed as follows: the poIFN-α14 and 17 subtypes had the greatest antiviral activities against VSV and PRV in PK-15 cells, poIFN-α1, 2, 3, and 8 exhibited lower biological activities, and poIFN-α4, 5, 6, 7, 9, 10, 11, 12, 13, and 16 had minimal or no effect in the tested target cell‒virus systems. Moreover, our studies demonstrated that the antiviral activity of IFN-α was positively correlated with the induction of IFN-stimulated genes, such as 2′–5′ oligoadenylate synthetase 1 (OSA1), interferon-stimulated gene 15 (ISG15), myxoma resistance protein 1 (Mx1), and protein kinase R (PKR). Thus, our experimental results provide important information about the antiviral functions and mechanism of poIFN-α. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15733149
- Volume :
- 29
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- International Journal of Peptide Research & Therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 162985214
- Full Text :
- https://doi.org/10.1007/s10989-023-10508-3