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Decreased Expression of Urethral Caveolin-1, -2, and -3 in the Rat Model of Overactive Bladder: Potential Mediator of Functional Interaction of Urethra and Urinary Bladder.
- Source :
-
International Neurourology Journal . Mar2023, Vol. 27 Issue 1, p47-54. 8p. - Publication Year :
- 2023
-
Abstract
- Purpose: To investigate the effect of detrusor overactivity (DO) on the urethral expression of caveolin (CAV)-1, -2, and -3 of urethra in an animal model of cyclophosphamide (CYP)-induced cystitis rat. Methods: Female Sprague-Dawley rats were divided into the control group (n=20) and the cystitis group (n=20). Cystitis was induced by intraperitoneal injection of CYP (200 mg/kg). An urodynamic study was done 3 days after the CYP injection to measure functional change of the urinary bladder and urethra. Cellular localization and expression of CAV-1, -2, and -3 in the rat urethra were determined by immunohistochemistry (IHC) and Western blot. Results: Urodynamic experiments demonstrated a decreased contraction interval in the cystitis group compared to the control (3.9±1.0 minutes vs. 6.6±1.2 minutes, P<0.05). Conversely, contraction pressure increased significantly in the cystitis group compared to the control (22.4±0.7 mmHg vs. 11.5±0.4 mmHg, P<0.05). The urethral pressure was decreased in the cystitis group compared to the control (4.05±2.5 mmHg vs. 5.8±2.8 mmHg, P<0.05). The IHC and Western blot data showed that CAV-1, -2, and -3 expression decreased significantly in the cystitis group compared control group (P<0.05). Conclusions: The decreased urethral CAV-1, -2, and -3 in the DO rats suggests that CAVs might be related with the functional change of urethra in association with DO of urinay bladder. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BLADDER
*OVERACTIVE bladder
*URETHRA
*CAVEOLINS
*ANIMAL disease models
*URODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 20934777
- Volume :
- 27
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- International Neurourology Journal
- Publication Type :
- Academic Journal
- Accession number :
- 163009989
- Full Text :
- https://doi.org/10.5213/inj.2244236.118