低温胁迫对 4 种蔷薇属植物的生理指标及 HSP90 基因表达的影响.

To study the difference of cold resistance of four plants of Rosa(R. lasiosepala, R. odorata. var. gigantea, R. weisiensis, R. lichiangensis), and screen out the varieties with strong cold resistance. In this study, a low temperature incubator was used to conduct different gradients of low temperature stress(0, -5, -10, -15 ℃), the soluble sugar content, soluble protein content, malondialdehyde(MDA) content, proline(Pro) content, SOD activity, POD activity, and expression difference of coldresistance-related regulatory gene HSP90 were measured. The results showed that under low temperature stress, the soluble protein content, soluble sugar content, free proline content, malondialdehyde content, SOD activity, and POD activity in the leaves of four plants of Rosa showed a gradually increasing trend with aggravation of low temperature stress. The analysis of the differential expression of HSP90 gene of four plants of Rosa showed that the relative expression of HSP90 gene showed a trend of gradual increase with aggravation of low temperature stress. The expression of HSP90 gene was the highest in R. lasiosepala under 0, - 5, - 10, -15 ℃ low temperature stress, indicating that there were differences in cold resistance of four plants of Rosa, among which R. lasiosepala had better cold resistance. [ABSTRACT FROM AUTHOR]