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Real-Time Dissection of the Transportation and miRNA-Release Dynamics of Small Extracellular Vesicles.
- Source :
-
Advanced Science . 3/3/2023, Vol. 10 Issue 7, p1-16. 16p. - Publication Year :
- 2023
-
Abstract
- Extracellular vesicles (EVs) are cell-derived membrane-enclosed structures that deliver biomolecules for intercellular communication. Developing visualization methods to elucidate the spatiotemporal dynamics of EVs' behaviors will facilitate their understanding and translation. With a quantum dot (QD) labeling strategy, a single particle tracking (SPT) platform is proposed here for dissecting the dynamic behaviors of EVs. The interplays between tumor cell-derived small EVs (T-sEVs) and endothelial cells (ECs) are specifically investigated based on this platform. It is revealed that, following a clathrin-mediated endocytosis by ECs, T-sEVs are transported to the perinuclear region in a typical three-stage pattern. Importantly, T-sEVs frequently interact with and finally enter lysosomes, followed by quick release of their carried miRNAs. This study, for the first time, reports the entire process and detailed dynamics ofT-sEV transportation and cargo-release in ECs, leading to better understanding of their proangiogenic functions. Additionally, the QD-based SPT technique will help uncover more secrets of sEV-mediated cell-cell communication. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21983844
- Volume :
- 10
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Advanced Science
- Publication Type :
- Academic Journal
- Accession number :
- 163274871
- Full Text :
- https://doi.org/10.1002/advs.202205566