Hypochlorous acid-activated near-infrared fluorescent probe for in vivo/exogenous detection and dairy toxicity evaluation.

In this study, we designed and synthesized a NIR probe, named W-1a derivatives for specific detection of ClO−. The probe exhibited dual fluorescence and colorimetric sensing with a response time of <1 min and a detection limit of 0.15 μM. Additionally, the probe was successfully applied for fluorescence imaging of ClO− at the cellular level and its endogenous/exogenous detection in zebrafish and dairy products. [Display omitted] • Development of a near-infrared fluorescent probe for hypochlorite detection. • Highly selective and sensitive responses with a low limit of detection. • The ClO− probe mediated for fluorescence imaging of HeLa cells and zebrafish and evaluating the toxicity of dairy products. Oxidative stress has been reported to be closely associated with many diseases, and an excessive overdose of hypochlorite (ClO−) can also induce stress-related diseases. In this study, we designed and synthesized a NIR probe, named W-1a based on computational analysis of DCM (4-(Dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran) derivatives for specific detection of ClO−. The probe exhibited dual fluorescence and colorimetric sensing with a response time of <1 min and a detection limit of 0.15 μM. Additionally, the probe was successfully applied for fluorescence imaging of ClO− at the cellular level and ebrafish endogenous/exogenous ClO− assay and dairy toxicity assessment. Thus, we present a potential method for developing an efficient and reliable detection of ClO− in early stage using near-infrared dyes. [ABSTRACT FROM AUTHOR]