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Production and Characterization of Monoclonal Antibodies Against Structural Proteins of Hirame Novirhabdovirus.
- Source :
-
Monoclonal Antibodies in Immunodiagnosis & Immunotherapy . Apr2023, Vol. 42 Issue 2, p53-58. 6p. - Publication Year :
- 2023
-
Abstract
- Hirame novirhabdovirus (HIRRV) is a significant viral pathogen of Japanese flounder (Paralichthys olivaceus). In this study, seven monoclonal antibodies (mAbs) against HIRRV (isolate CA-9703) were produced and characterized. Three mAbs (1B3, 5G6, and 36D3) were able to recognize nucleoprotein (N) (42 kDa) and four mAbs (11-2D9, 15-1G9, 17F11, and 24-1C6) recognized matrix (M) protein (24 kDa) of HIRRV. Western blot, Enzyme-linked immunosorbent assay, and indirect fluorescent antibody technique (IFAT) results indicated that the developed mAbs were specific to HIRRV without any cross-reactivity against other different fish viruses and epithelioma papulosum cyprini cells. All the mAbs comprised IgG1 heavy chain and κ light chain except 5G6, which has a heavy chain of IgG2a class. These mAbs can be very useful in development of immunodiagnosis of HIRRV infection. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21679436
- Volume :
- 42
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Monoclonal Antibodies in Immunodiagnosis & Immunotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 163369886
- Full Text :
- https://doi.org/10.1089/mab.2022.0040