Effect Of Mangosteen Peel Extract On Increasing The Number Of Endocrine Cells In Pancreas Langerhans Islands And Decreasing Tumor Necrosis Factor Alfa In Streptozotocin-Induced Male Rats.

This study aims to analyze the effect of mangosteen peel on increasing the number of pancreatic islets in Langerhans endocrine cells and decreasing TNF-α in male white rats induced by streptozotocin. The research conducted was laboratory experimental in nature. The research design used is a Randomized Post Test Only Control Group Design. The samples were 24 male white rats of the Wistar strain. Rats were grouped into six groups: negative control group (KN), positive control STZ (K1), positive control metformin synthetic drug therapy (K2), mangosteen rind extract therapy dose of 200 mg/kgbb (K3), a dose of 400 mg/kgbb (K4), a dose of 600 mg/kgbb (K5). Administration of therapy for 14 days orally. Pancreatic organs were examined by HE and IHC staining. Pancreatic histopathological observation and TNF-α expression were carried out microscopically. Statistical analysis using the Kolmogorov-Smirnov, ANOVA test, and Pearson's correlation. The results of the effect of mangosteen peel extract can increase the proliferation of endocrine cells in the islets of Langerhans, at a dose of 600 mg/kg BW/day. While the decrease in TNF-α expression was shown by all doses of mangosteen peel extract and appeared to be significantly different when compared to the K1 group, namely the STZ-induced group without any therapy. Pearson's correlation results show that there is an inverse relationship between the number of endocrine cells and TNF-α expression. The conclusion is that administration of mangosteen peel extract can increase the number of pancreatic islets of Langerhans endocrine cells and decrease Tumor Necrosis Factor Alpha in streptozotocin-induced male white rats. Suggestions need to do research on anti-inflammatory cytokines that occur in endocrine cells of the islets of Langerhans due to streptozotocin-induced mangosteen peel extract in rats so that there is a balance with pro-inflammatory cytokines. [ABSTRACT FROM AUTHOR]